Food Safety & Quality Newsletter April 2021 Edition

Mycotoxins are naturally occurring toxins produced by molds or fungi, most often developed due to extreme weather events. To help you detect mycotoxins and aflatoxins in grain and cereals, PerkinElmer has developed a wide range of test kits and strip tests able to perform key protective measures and ensure grains are free of harmful toxins before entering the food supply.

The MaxSignal® Aflatoxin B1 ELISA Kit is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin B1 in milk, milk powder, chicken, pork, beef, seed, feed, cereal, edible oils, pastries, and peanut butter.

This work shows a reliable, sensitive and selective method for multi-mycotoxin determination in food samples using a PerkinElmer QSight 220 LC-MS/MS system. The method has been validated in eight different matrices and can be used in routine testing labs to meet regulatory requirements.

MaxSignal HTS Nitrofurans and Chloramphenicol ELISA Kits assays are designed and developed specifically for the aquaculture industry, delivering a simple, simultaneous, 5-in-1 sample preparation method for AOZ, AMOZ, SEM and AHD Nitrofurans as well as Chloramphenicol.

Being able to accurately and rapidly test raw meat, and specifically beef, for bacterial contaminants such as E. coli is necessary to ensure those food products are safe to consume. The Solus One E. coli O157 is an extremely sensitive and specific enhanced ELISA for the screening of large samples of raw beef to detect E. coli O157:H7 with time to results within 12 - 14 hours. With results one day faster than other molecular or culture methods, the Solus One assay is efficient.

In this application note, a robust, high-throughput method for the analysis of 73 veterinary drugs in chicken tissue is presented. The method utilizes a fully automated solid phase micro-extraction (SPME) sample preparation technique, with subsequent analysis on a QSight® UHPLC/MS/MS.

High Throughput Screening of Aflatoxin M1 in Milk Samples by Automated ELISA Platform In the following study we demonstrate the accuracy and precision of the MaxSignal® Aflatoxin M1 ELISA kit (Cat# FOOD-1060-05) at the EU MRL of 0.05 ppb and the US MRL of 0.5 ppb. We also provide data showing the limit of detection (LOD) of the kit. Further, we show the variability between performance of manual ELISA and DS2 automated ELISA platform.

Aflatoxin B1 is often found in animal feeds such as corn, cottonseed cake, groundnut meal, and other agricultural commodities. When these contaminated feeds are ingested by milk-producing animals, such as cattle, aflatoxin B1 can be converted to aflatoxin M1 (a hydroxy derivate of aflatoxin B1) by oxidation metabolism, and then excreted in milk.

Solus One Salmonella has been validated by ISO 16140 from ANFOR and AOAC OMA for the decection of Salmonella in 24 hours for various Dairy matrcies. Such as instant non-fat dry milk powder, pasteurized milk, milk cheese and milk based products.

PerkinElmer’s ONE Pesticide420™ ISO 17034 CRM Reagent Kit (the “Kit”) saves laboratories time and energy by providing users with pre-formatted pesticide and mycotoxin CRM calibration reagents, QC standards and lab consumables from a single source. Ready to use out of the box without any additional dilution or mixing steps each Kit is designed to test up to 500 samples and help laboratories meet state testing requirements in states that include some or all of the analytes found in the California and Oregon cannabis testing regulations as of March, 2021.

Most laboratories require multiple sample preparation steps to perform their pesticide residue assay. This workflow is time-consuming, prone to errors due to the human variability, and poorly suited to high volume cannabis and hemp testing labs. To dramatically improve the labs "Turn Around Time" and data quality, PKI has developed a fully automated sample preparation workflow and LC/MSMS analytical solution to meet State and Country cannabis compliance needs.

Accurate identification and quantification of cannabinoid content is a critical step in the process to bringing safe and high quality cannabis flower and fortified products to market. Fast and efficient liquid chromatography methods for the determination of cannabinoid content are need to satisfy both regulatory and consumer demands.

Solus One Salmonella has been validated by ISO 16140 from ANFOR and AOAC OMA for the decection of Salmonella for a range of ready to eat food and enviromental samples.

Solus One Salmonella was compared to FDA Chapter 5 and ISO 6579-1:2017 for 375g samples of cocoa powder, cocoa liquor, and milk chocolate and was shown to have a performance equivalent to that of the reference methods.

Avio 220 Max results in unrivaled sensitivity for an ICP-OES instrument, which is important when measuring low-sensitivity analytes, such as Cl. The optical design also allows for simultaneous acquisition of all data points around the analyte peak at the same time as the analyte, resulting in stable, reproducible backgrounds.

Pesticides are utilized in a number of agricultural applications to prevent, destroy, or control harmful organisms or diseases, as well as protect plants or plant products during production, storage and transport.

In a fast-paced food testing environment, it’s critical to process samples quickly and efficiently. Providing rapid, accurate results allows production facilities to act quickly if E. coli O157 is detected.

The ONE Pesticide420™ Reagent Kit is a first of its kind in the industry that offers the lab analyst the most efficient sample preparation possible for pesticide residue analysis in cannabis and hemp samples due to being pre-mixed and pre-formulated ISO 17034:2016 certified reagents.

To protect the health of allergic consumers from the inadvertent or intentional contamination by allergens, different analytical methods for the sensitive detection of allergens in food were developed over the years. In this work we present the performance of a targeted method using the QSight 220 Liquid Chromatography Triple Quadrupole Mass Spectrometer (LC-MS/MS) for the simultaneous quantification of milk and egg allergens in thermally processed bakery products.

We are please to present you our new Validated Multiclass-Multianalyte Method for Mycotoxin Determination in Various Food Matrices by Coupling Immunoaffinity Column Sample Clean-up with QSight LC/MS/MS.

Accurate calibrations play a vital role in NIR analysis. With emphasis on food, feed, and related agricultural industries.

PerkinElmer is pleased to announce an upcoming bi-lingual webinar. Presentations will be live and include immediate translation. A question and answer session will follow. PerkinElmer is introducing end-to-end workflow solution that includes the easiest and comprehensive multiplexed sample preparation for Nitrofurans and Chloramphenicol in shrimp samples, and an automated high-throughput detection platform.

Check out our on demand webinar to learn about our Glutomatic 2000 system and how to measure Gluten quantity and quality.

Our ongoing mission to bring together industry professionals for thoughtful insight as discussion, we will include a 30 minute lunch open Q&A session mid program.  Additionally, as a welcome and a thank you for joining us, participants of the webinar symposium will receive a $20 GrubHub eGiftCard towards a future meal emailed to them during the presentations.

The state of California currently presents the most challenging requirements for pesticide analysis in the United States, with 66 pesticides and 5 mycotoxins regulated by the Bureau of Cannabis Control. In this talk, we will discuss the quantification of these analytes in challenging matrix types, cannabis flower and cannabis concentrates (which can contain 50-95% cannabinoids).