Metanephrine and Normetanephrine Quantification by SPE-LC-MS/MS

Introduction

Measurement of metanephrines and normetanephrine in plasma is challenging due to the low physiological concentrations, their hydrophilic nature1, and time consuming traditional sample preparation. With use of the PerkinElmer QSight® 220 triple quadrupole mass spectrometer, LC-MS/MS analysis was performed using the recently advanced solid phase extraction (SPE) sorbent technology (“load, wash, elute”) method protocol. As a result low levels of ME and NOR are detectable in plasma with short sample preparation and LC run time. This LC-MS/MS method provides a fast, sensitive, accurate, and reproducible solution for the analysis of ME and NOR in plasma.