This poster shows how a single method workflow has been developed and validated following AOAC SMPR and USP <233> requirements to validate metals beyond As, Cd, Pb, and Hg. This work presents the quantitation limits, repeatability, and accuracy of this method. In addition, it also investigates and discusses the influences of rare earth elements in cannabis.

To ensure food safety and enforce regulations, reliable analytical methods are needed for monitoring drugs in different sample matrices. In this study, a sensitive method was developed for the analysis of 47 antibiotics and veterinary drugs in animal feed and animal tissue samples.

Isovaleric Acidemia (IVA) is an autosomal recessive disorder of leucine metabolism. IVA is caused by Isovaleryl-CoA dehydrogenase (IVD) gene mutations, and phenotypic abnormalities seen are due to the accumulation of isovaleric acid, which is toxic to the central nervous system. IVA newborn screening is commonly based on the measurement of increased isovalerylcarnitine (C5) concentration in blood. By direct flow injection analysis tandem mass spectrometry (MS/MS) assay without liquid chromatography (LC) separation, this specific biomarker cannot be however distinguished from the three other C5 isobarsvaleryl-, pivaloyl-, and 2-methylbutyrylcarnitine. Therefore, to allow more specific C5 marker measurement and to reduce false positive rates in the IVA screening, we detail here a gradient LC-MS/MS method, which is capable of separating all four isobaric C5 acylcarnitines in dried blood spot (DBS).

Ingestion of cyanotoxins causes negative human health effects such as liver inflammation and hemorrhage, vomiting and diarrhea, and abdominal pain. The US EPA has a health advisory specifying the maximum recommended level of microcystins for all ages.

EPA Method 544 uses solid phase extraction liquid chromatography tandem mass spectrometry (SPE-LC/MS/MS) for quantitation of 6 microcystins and nodularin in drinking water samples. A PerkinElmer QSight®LX50 UHPLC system coupled with a PerkinElmer QSight®200 series triple quadrupole mass spectrometer was used to quantitate the microcystins and nodularin and meet EPA method 544 requirements.

The QSight triple quadrupole mass spectrometer from PerkinElmer has a unique feature in that it has two standalone ionization sources. This gives the user maximum flexibility by enabling them to switch easily between electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI). Moreover, there is no need to manually change anything on the instrument and no tools are required. The examples presented here will demonstrate two possible scenarios a user could encounter during method development when dealing with difficult compounds.

Regulatory water directives from around the globe have implemented environmental quality standards for drinking water. One class of compounds included are the polyaromatic hydrocarbons (PAH) that are of concern because of their potential adverse effects to our health. In the present work we demonstrate a robust and highly sensitive method using online SPE-LC/MS/MS to quantify the five PAHs (Benzo(a)pyrene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(ghi)perylene, indeno(1,2,3-cd) pyrene) listed in the latest EU drinking water directive.