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Presented in a Q&A format, this session of our LC learning series will explore some of the most common issues/problems encountered in the HPLC lab. As the most important diagnostic tool for HPLC is monitoring the pump pressure, several examples of pressure issues will be discussed. Other problems, such as retention time and peak area irreproducibility, and detector baseline noise or drift will be also presented. The aim of this session is to provide some basic troubleshooting fundamentals to build confidence in recognizing when issues are occurring and how to resolve them to improve instrument uptime and laboratory productivity.